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Identification and characterization of the Arabidopsis thaliana chloroplast DNA region containing the genes psbA, trnH and rps19? by Karsten Liere; Maya Kestermann; Ulrike Mller; Gerhard Link is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification and characterization of the Arabidopsis thaliana chloroplast DNA region containing the genes psbA, trnH and rps19? about?

A 1887-nucleotide chloroplast-DNA region from Arabidopsis thaliana was analyzed. It contains the conserved genes psbA for the precursor of the D1 reaction-centre protein of photosystem II, trnH for tRNAHis, and rps19' for the 6.8-kDa protein of the small ribosomal subunit. Northern hybridization and RNase protection experiments suggest co-transcription of a minor RNA fraction over the full lengths of psbA and the preceding trnK-UUU gene, but not including downstream trnH sequences. In front of the mapped 5' end of the major 1.2-kb psbA transcript is a DNA region that shows the typical architecture of a psbA promoter, consisting of the prokaryotic-type '-35' and '-10' elements as well as the eukaryotic-type 'TATA' motif. The common 3' end of psbA transcripts seems to be located immediately after a stem-loop structure downstream from the coding region.

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Author

Karsten Liere; Maya Kestermann; Ulrike Mller; Gerhard Link

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0172-8083)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)