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Oriented Immobilization of Epidermal Growth Factor Onto Culture Substrates for the Selective Expansion of Neural Stem Cells by Tadashi Nakaji-Hirabayashi; Koichi Kato; Yusuke Arima; Hiroo Iwata is a Engineering article available to read on EtoBox.

What is Oriented Immobilization of Epidermal Growth Factor Onto Culture Substrates for the Selective Expansion of Neural Stem Cells about?

To develop a culture substrate that allows efficient expansion of neural stem cells (NSCs), epidermal growth factor (EGF) was immobilized onto the Ni(II)-chelated surface of a glass-based substrate through coordination of Ni(II) to the histidine tag that was fused to the C-terminal of EGF using recombinant technology. For the preparation of the nickel-chelated surface, a thin gold layer was deposited to the glass surface, and then the self-assembled monolayer of alkanethiol terminated with trivalent carboxylic acids was formed on gold and chelated with Ni(II) ions. In the preparation of a monolayer, triethylene glycol-terminated alkanethiol was mixed with carboxylic acid-terminated alkanethiol at various compositions in order to reduce the non-specific adsorption of EGF. The surface analysis of the monolayers was performed by X-ray photoelectron spectroscopy, infrared reflection-absorption spectroscopy, and contact angle measurements. Surface plasmon resonance analyses and protein assays were performed for characterizing EGFimmobilized surfaces. The proliferation and differentiation of rat fetal NSCs were examined on the EGF-chelated substrates to assess quantitatively the effects

Who reads Oriented Immobilization of Epidermal Growth Factor Onto Culture Substrates for the Selective Expansion of Neural Stem Cells?

Author

Tadashi Nakaji-Hirabayashi; Koichi Kato; Yusuke Arima; Hiroo Iwata

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)