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A Rat Brain cDNA Encodes Enzymatically Active GABA Transaminase and Provides a Molecular Probe for GABA‐Catabolizing Cells by Lali K. Medina‐Kauwe; Niranjala J. K. Tillakaratne; Jang‐Yen Wu; Allan J. Tobin is a Neuroscience article available to read on EtoBox.
What is A Rat Brain cDNA Encodes Enzymatically Active GABA Transaminase and Provides a Molecular Probe for GABA‐Catabolizing Cells about?
Abstract: cDNAs encoding γ‐aminobutyric acid aminotransferase (GABA‐T) were isolated from a λZAP rat hippocampal cDNA expression library by two independent cloning methods, immunological screening with an antimouse GABA‐T antibody and plaque hybridization with a GABA‐T cDNA probe derived by polymerase chain reaction. We have produced enzymatically active GABA‐T from a rat brain cDNA containing the full‐length GABA‐T coding region. Our rat brain GABA‐T cDNAs hybridize to mRNAs in brain and peripheral tissues, including liver, kidney, and testis. We have also detected GABA‐T mRNA in GABAergic cells of rat cerebellar cortex by in situ hybridization. Our rat brain GABA‐T probe hybridizes to Purkinje, basket, stellate, and Golgi II cells, the same GABAergic neurons previously shown to contain glutamate decarboxylase GAD~85~ and GAD~87~.
Who reads A Rat Brain cDNA Encodes Enzymatically Active GABA Transaminase and Provides a Molecular Probe for GABA‐Catabolizing Cells?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Lali K. Medina‐Kauwe; Niranjala J. K. Tillakaratne; Jang‐Yen Wu; Allan J. Tobin
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1994
- Language
- EN
- Field
- Neuroscience (Life Sciences)