About this Biochemistry, Genetics and Molecular Biology article

Identification and Expression Analysis of Genes Associated with Bovine Blastocyst Formation by Karen Goossens; Ann Van Soom; Mario Van Poucke; Leen Vandaele; Jo Vandesompele; Alex Van Zeveren; Luc J Peelman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Background Normal preimplantation embryo development encompasses a series of events including first cleavage division, activation of the embryonic genome, compaction and blastocyst formation. First lineage differentiation starts at the blastocyst stage with the formation of the trophectoderm and the inner cell mass. The main objective of this study was the detection, identification and expression analysis of genes associated with blastocyst formation in order to help us better understand this process. This information could lead to improvements of __in vitro__ embryo production procedures. ## Results A subtractive cDNA library was constructed enriched for transcripts preferentially expressed at the blastocyst stage compared to the 2-cell and 8-cell stage. Sequence information was obtained for 65 randomly selected clones. The RNA expression levels of 12 candidate genes were determined throughout 3 stages of preimplantation embryo development (2-cell, 8-cell and blastocyst) and compared with the RNA expression levels of __in vivo__ "golden standard" embryos using real-time PCR. The RNA expression profiles of 9 (75%) transcripts (__KRT18__, __FN1__, __MYL6__, __ATP1B3__, __FTH1__,

Author

Karen Goossens; Ann Van Soom; Mario Van Poucke; Leen Vandaele; Jo Vandesompele; Alex Van Zeveren; Luc J Peelman

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-213X)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)