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Expression of Binding Sites for β Chemokines on Human Astrocytes by Anuska V. Andjelkovic; Danielle Kerkovich; John Shanley; Lynn Pulliam; Joel S. Pachter is a Neuroscience article available to read on EtoBox.

What is Expression of Binding Sites for β Chemokines on Human Astrocytes about?

Astrocytes are major sources of chemokines and are thus critical effectors of central nervous system (CNS) inflammation. However, it is as yet unclear whether these cells, like leukocytes, also possess receptors for chemokines (CCRs). To address this issue, we utilized a novel fluorescence approach to detect qualitatively and quantitatively binding sites for biotinylated derivatives of the ␤ chemokines monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-1␣ (MIP-1␣) on cultured human fetal astrocytes. Both chemokines were found to bind to the surface of astrocytes in a specific and saturable manner and with the high-affinity typical of these chemokines' binding to leukocyte CCRs. Binding of labeled MCP-1 and of labeled MIP-1␣ was antagonized by the respective unlabeled homologue but not by the unlabeled heterologous chemokine. Binding of labeled MCP-1 was also inhibited by unlabeled MCP-3, both of which are ligands for CCR2. In a parallel manner, binding of labeled MIP-1␣ was first shown to be attenuated by unlabeled RANTES, both of which recognize CCR1 and CCR5, and then separately antagonized by MCP-3 and MIP-1␤, which bind to CCR1 and CCR5, respectively. Fi

Who reads Expression of Binding Sites for β Chemokines on Human Astrocytes?

Author

Anuska V. Andjelkovic; Danielle Kerkovich; John Shanley; Lynn Pulliam; Joel S. Pachter

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

1999

Language

EN

Field

Neuroscience (Life Sciences)