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Differentiation of Mouse Embryonic Stem Cells Into Hepatocytes Induced by a Combination of Cytokines and Sodium Butyrate by Mingming Zhou; Ping Li; Li Tan; Su Qu; Qi-Long Ying; Houyan Song is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract There is increasing evidence to suggest that embryonic stem cells (ESCs) are capable of differentiating into hepatocytes in vitro. In this study, we used a combination of cytokines and sodium butyrate in a novel three‐step procedure to efficiently direct the differentiation of mouse ESCs into hepatocytes. Mouse ESCs were first differentiated into definitive endoderm cells by 3 days of treatment with Activin A. The definitive endoderm cells were then differentiated into hepatocytes by the addition of acidic fibroblast growth factor (aFGF) and sodium butyrate to the culture medium for 5 days. After 10 days of further in vitro maturation, the morphological and phenotypic markers of hepatocytes were characterized using immunohistochemistry, immunoblotting, and reverse transcription‐polymerase chain reaction (RT‐PCR). Furthermore, the cells were tested for functions associated with mature hepatocytes, including glycogen storage and indocyanine green uptake and release, and the ratio of hepatic differentiation was determined by counting the percentage of albumin‐positive cells. In the presence of medium containing cytokines and sodium butyrate, numerous epithelial cells resem

Who reads Differentiation of Mouse Embryonic Stem Cells Into Hepatocytes Induced by a Combination of Cytokines and Sodium Butyrate?

Author

Mingming Zhou; Ping Li; Li Tan; Su Qu; Qi-Long Ying; Houyan Song

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)