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Can I read Isolation of Nitric Oxide Synthase from Human Platelets on EtoBox?
Isolation of Nitric Oxide Synthase from Human Platelets by Arumugam Muruganandam; Bulent Mutus is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Isolation of Nitric Oxide Synthase from Human Platelets about?
We are reporting a distinct constitutive isoform of nitric oxide synthase that has been purified to homogeneity from human platelet cytosolic fractions. Purification involved ultra centrifugation at 100000 x g followed by two sequential affinity chromatography procedures: adenosine 2',5'-bisphosphate (2 ',5 '-ADP)-Sepharose and calmodulin Sepharose 4B. Purified enzyme appeared as a single band (~ 80 kDa) under denaturing condition (SDS-PAGE). The native enzyme appears to be dimeric, since its molecular weight estimated by gel filtration was ~ 150 kDa. Enzyme activity was dependent on L-arginine, NADPH and (6R)-5,6,7,8-tetrahydro-L-biopterine. Partially purified platelet NOS (100 000 x g supernatant) activity was sensitive to calmodulin antagonists and to the N'°-Monomethyl-L-arginine, a substrate analog of L-arginine.
Who reads Isolation of Nitric Oxide Synthase from Human Platelets?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Arumugam Muruganandam; Bulent Mutus
- Publisher
- Elsevier BV
- Published
- 1994
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)