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Characterization of cyanobacterial β‐carotene ketolase and hydroxylase genes in Escherichia coli , and their application for astaxanthin biosynthesis by Mark A. Scaife; Adam M. Burja; Phillip C. Wright is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Carotenoid biosynthesis is highly conserved and well characterized up to the synthesis of β‐carotene. Conversely, the synthesis of astaxanthin from β‐carotene is less well characterized. Regardless, astaxanthin is a highly sought natural product, due to its various industrial applications and elevated antioxidant capacity. In this article, 12 β‐carotene ketolase and 4 β‐carotene hydroxylase genes, isolated from 5 cyanobacterial species, are investigated for their function, and potential for microbial astaxanthin synthesis. Further, this in vivo comparison identifies and applies the most promising genetic elements within a dual expression vector, which is maintained in __Escherichia coli__. Here, combined overexpression of individual β‐carotene ketolase and β‐carotene hydroxylase genes, within a β‐carotene accumulating host, enables a 23.5‐fold improvement in total carotenoid yield (1.99 mg g^−1^), over the parental strain, with >90% astaxanthin. Biotechnol. Bioeng. 2009;103: 944–955. © 2009 Wiley Periodicals, Inc.

Who reads Characterization of cyanobacterial β‐carotene ketolase and hydroxylase genes in Escherichia coli , and their application for astaxanthin biosynthesis?

Author

Mark A. Scaife; Adam M. Burja; Phillip C. Wright

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)