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This document discusses the production of chymosin, the main coagulating enzyme in rennet, through recombinant methods. It describes how chymosin is normally sourced from calf stomachs but there is increasing demand and shortage of supply. The document outlines how the calf chymosin gene was expressed in Kluyveromyces lactis yeast to produce recombinant chymosin as K. lactis is a nontoxicogenic yeast approved by the FDA for production of heterologous proteins. Disrupting the PMR1 gene in K. lactis was found

Author

Enzo Taping

Language

EN