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In Vivo Biosynthesis of Clathrin and Other Coated Vesicle Proteins from Rat Liver by L. Ross Pierce; Chiara Zurzolo; Harold Edelhoch is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A biosynthetic study of rat liver coated vesicle (CV) proteins was undertaken by using in vivo labeling with L-[35S]methionine. CVs were isolated and purified by using standard procedures and characterized by electron microscopy, sedimentation, and sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by fluorography, or by gel slicing and liquid scintillation counting. After 5 ?h min of labeling (the earliest time examined), incorporation of radioactive clathrin heavychain (180-kD (kilodalton)) subunits as well as a 90-kD CV-associated protein into purified CVs was demonstrated. The level of labeled 180-kD clathrin in coated vesicles increased rapidly during the first 2 hr of labeling and then continued to rise at a slower rate between 4 and 16 hr. This slow accumulation of labeled clathrin heavy chains in the CV pool may reflect early compartmental sequestration of a fraction of newly synthesized clathrin with delayed assembly into free CVs. By 16 hr of labeling, clathrin 180-kD chains and the 90-kD CVassociated protein accounted for approximately 48 and 26 % , respectively, of the radioactivity in all CV proteins. Two proteins of MW, 68 kD and 53 kD showed marked de

Who reads In Vivo Biosynthesis of Clathrin and Other Coated Vesicle Proteins from Rat Liver?

Author

L. Ross Pierce; Chiara Zurzolo; Harold Edelhoch

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1986

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)