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Expression of Uncoupling Protein-3 in Subsarcolemmal and Intermyofibrillar Mitochondria of Various Mouse Muscle Types and Its Modulation by Fasting by Maria Jimenez; Cedric Yvon; Lorenz Lehr; Bertrand Léger; Patrick Keller; Aaron Russell; Françoise Kuhne; Pierre Flandin; Jean-Paul Giacobino; Patrick Muzzin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Expression of Uncoupling Protein-3 in Subsarcolemmal and Intermyofibrillar Mitochondria of Various Mouse Muscle Types and Its Modulation by Fasting about?
Uncoupling protein‐3 (UCP3) is a mitochondrial inner‐membrane protein abundantly expressed in rodent and human skeletal muscle which may be involved in energy dissipation. Many studies have been performed on the metabolic regulation of UCP3 mRNA level, but little is known about UCP3 expression at the protein level. Two populations of mitochondria have been described in skeletal muscle, subsarcolemmal (SS) and intermyofibrillar (IMF), which differ in their intracellular localization and possibly also their metabolic role. To examine if UCP3 is differentially expressed in these two populations and in different mouse muscle types, we developed a new protocol for isolation of SS and IMF mitochondria and carefully validated a new UCP3 antibody. The data show that the density of UCP3 is higher in the mitochondria of glycolytic muscles (tibialis anterior and gastrocnemius) than in those of oxidative muscle (soleus). They also show that SS mitochondria contain more UCP3 per mg of protein than IMF mitochondria. Taken together, these results suggest that oxidative muscle and the mitochondria most closely associated with myofibrils are most efficient at producing ATP. We then determined the e
Who reads Expression of Uncoupling Protein-3 in Subsarcolemmal and Intermyofibrillar Mitochondria of Various Mouse Muscle Types and Its Modulation by Fasting?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Maria Jimenez; Cedric Yvon; Lorenz Lehr; Bertrand Léger; Patrick Keller; Aaron Russell; Françoise Kuhne; Pierre Flandin; Jean-Paul Giacobino; Patrick Muzzin
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)