Opening book details…
Can I read Automated 96-Well Solid Phase Extraction for the Determination of Doramectin in Cattle Plasma on EtoBox?
Automated 96-Well Solid Phase Extraction for the Determination of Doramectin in Cattle Plasma by A.C Harrison; D.K Walker is a Chemistry article available to read on EtoBox.
What is Automated 96-Well Solid Phase Extraction for the Determination of Doramectin in Cattle Plasma about?
Automated standard and sample preparation have been coupled with 96-well solid phase extraction (SPE) technology to produce a cost effective, high throughput system for the analysis of drugs in biological media. The system was originally designed using the Packard Multiprobe 104DTTM robotic sample processor (RSP) to improve throughput for the assay of doramectin in cattle plasma, and the assay has since been validated (0.5 -100 ng ml -1 ) using the Tecan Genesis RSP 150/8TM. The robotic processor conducts all liquid handling procedures involved in sample extraction. These comprise preparation of calibration standards in plasma, dispensing and diluting of plasma samples and addition of internal standard. In addition, the robot primes the 96-well SPE block, applies calibration standards and samples, draws the mixtures through the 96-well SPE block, and finally washes the block ready for manual elution. The doramectin assay involves high-performance liquid chromatography (HPLC) with fluorescence detection, and requires the sample extracts to be derivatised prior to analysis. The derivatisation procedure is performed manually in situ in the polypropylene deep 96-well block into which t
Who reads Automated 96-Well Solid Phase Extraction for the Determination of Doramectin in Cattle Plasma?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- A.C Harrison; D.K Walker
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)
- Published
- 1998
- Language
- EN
- Field
- Chemistry (Physical Sciences)