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A study investigated the effects of high-concentrate (HC) diets on milk fat synthesis in lactating dairy cows, revealing that such diets lead to a decline in milk fat due to altered liver lipid metabolism. The HC diet increased glucagon levels and activated the AMPK-PPARα signaling pathway, which reduced plasma concentrations of nonesterified fatty acids (NEFA) and affected milk composition negatively. Results indicate that long-term HC feeding is detrimental to cow health and milk quality, emphasizing the
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