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This document describes a method for determining the molecular weight distribution and average molecular weights of oligosaccharides using HPLC. The method involves derivatizing oligosaccharide samples with 4-aminobenzoic acid ethyl ester (ABEE) via reductive amination. The ABEE-derivatized oligosaccharides are then separated by HPLC using a C18 column with a mobile phase of high water content. Comparison of the experimental results to theoretical values for a synthetic oligosaccharide mixture showed good a

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TaurusVõ

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EN