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Can I read Methionine-producing Tumor Micro(be) Environment Fuels Growth of Solid Tumors on EtoBox?
Methionine-producing Tumor Micro(be) Environment Fuels Growth of Solid Tumors by Alexis A. Vega; Erin A. Marshall; Avery J. C. Noonan; Fernando Sergio Leitao Filho; Julia Yang; Greg L. Stewart; Fraser D. Johnson; Emily A. Vucic; Michelle E. Pewarchuk; Parag P. Shah; Brian F. Clem; Corey Nislow; Stephen Lam; William W. Lockwood; Steven J. Hallam; Janice M. Leung; Levi J. Beverly; Wan L. Lam is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Methionine-producing Tumor Micro(be) Environment Fuels Growth of Solid Tumors about?
## Background Recent studies have uncovered the near-ubiquitous presence of microbes in solid tumors of diverse origins. Previous literature has shown the impact of specific bacterial species on the progression of cancer. We propose that local microbial dysbiosis enables certain cancer phenotypes through provisioning of essential metabolites directly to tumor cells. ## Methods 16S rDNA sequencing of 75 patient lung samples revealed the lung tumor microbiome specifically enriched for bacteria capable of producing methionine. Wild-type (WT) and methionine auxotrophic (metA mutant) __E. coli__ cells were used to condition cell culture media and the proliferation of lung adenocarcinoma (LUAD) cells were measured using SYTO60 staining. Further, colony forming assay, Annexin V Staining, BrdU, AlamarBlue, western blot, qPCR, LINE microarray and subcutaneous injection with methionine modulated feed were used to analyze cellular proliferation, cell-cycle, cell death, methylation potential, and xenograft formation under methionine restriction. Moreover, C^14^-labeled glucose was used to illustrate the interplay between tumor cells and bacteria. ## Results/Discussion Our results show bacteria
Who reads Methionine-producing Tumor Micro(be) Environment Fuels Growth of Solid Tumors?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Alexis A. Vega; Erin A. Marshall; Avery J. C. Noonan; Fernando Sergio Leitao Filho; Julia Yang; Greg L. Stewart; Fraser D. Johnson; Emily A. Vucic; Michelle E. Pewarchuk; Parag P. Shah; Brian F. Clem; Corey Nislow; Stephen Lam; William W. Lockwood; Steven J. Hallam; Janice M. Leung; Levi J. Beverly; Wan L. Lam
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)