Opening book details…
Can I read A non-aqueous method for the sub-cellular fractionation of cotyledons from dormant seeds of Phaseolus vulgaris L. on EtoBox?
A non-aqueous method for the sub-cellular fractionation of cotyledons from dormant seeds of Phaseolus vulgaris L. by R. Begbie is a Agricultural and Biological Sciences article available to read on EtoBox.
What is A non-aqueous method for the sub-cellular fractionation of cotyledons from dormant seeds of Phaseolus vulgaris L. about?
A method is described for the sub-cellular fractionation of cotyledons from non-germinated Phaseolus vulgaris seeds in non-aqueous density gradients of potassium iodide in glycerol. The major organelles (protein bodies, cell walls and starch grains) are well resolved as adjudged by morphology and protein subunit patterns of gradient zones and are remarkably stable if water is rigorously excluded. The flexibility of the system is exploited in establishing the cytoplasmic location of the oligosaccharide fraction and in the large-scale preparative isolation of the sub-cellular components. The composition and morphology of the sub-cellular components are described and discussed.
Who reads A non-aqueous method for the sub-cellular fractionation of cotyledons from dormant seeds of Phaseolus vulgaris L.?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- R. Begbie
- Publisher
- Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)
- Published
- 1979
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)