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mESC Motoneuron Differentiation Guide by Oscar Marcelo Lazo is a document available to read on EtoBox.

What is mESC Motoneuron Differentiation Guide about?

This document provides instructions for differentiating mouse embryonic stem cells into motoneurons over 5 to 7 days. The process involves culturing cells in suspension to form embryoid bodies, then stimulating differentiation with retinoic acid and sonic hedgehog agonist before harvesting neurons and plating them on coated coverslips.

Author

Oscar Marcelo Lazo

Language

EN