Opening book details…
Can I read The Major Protamine from Stallion Sperm. Isolation and Amino-acid Sequence on EtoBox?
The Major Protamine from Stallion Sperm. Isolation and Amino-acid Sequence by Hubert AMMER; Agnes HENSCHEN is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The Major Protamine from Stallion Sperm. Isolation and Amino-acid Sequence about?
The major stallion protamine was isolated from sperm cell nuclei by extraction with 6M guanidine/5% mercaptoethanol, alkylation with 4-vinylpyridine and subsequent reversed-phase high-performance liquid chromatography. The primary structure of stallion protamine was determined by N-terminal se-quencing of the intact protein and of the fragments obtained from thermolysin cleavage of the S-pyridylethylated and from endoproteinase Lys-C cleavage of the S-aminoethylated protein. Stallion protamine consists of 49 amino-acid residues and shows 49% identity with all other sequenced mammalian type 1 protamines. ## Die Protamin-Hauptkomponente des Hengstes. Isolierung und Aminosäuresequenz Zusammenfassung: Die Protaminhauptkomponente des Hengstes wurde isoliert durch Extraktion der Spermienzellkerne mit 6M Guanidin/ 5% Mercaptoethanol, Alkylierung mit 4-Vinylpyridin und anschließende "reversed-phase"-Hochdruckflüssigkeitschromatographie. Die Primärstruktur des Hengstprotamins wurde bestimmt durch N-terminale Sequenzierung des intakten Proteins und der Fragmente, die durch Spaltung des S-pyridylethylierten Proteins mit Thermolysin und des S-aminoethylierten Proteins mit Endoproteinase Lys-C
Who reads The Major Protamine from Stallion Sperm. Isolation and Amino-acid Sequence?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hubert AMMER; Agnes HENSCHEN
- Published
- 1987
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)