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Identification of H-2Kb-restricted T-cell epitopes within the nucleocapsid protein of Hantaan virus and establishment of cytotoxic T-cell clones by Park, Jong Myun; Cho, Sung Yoo; Hwang, Yu Kyeong; Um, Sung Hee; Kim, Woo Jung; Cheong, Hong Seok; Byun, Si Myung is a Medicine article available to read on EtoBox.
What is Identification of H-2Kb-restricted T-cell epitopes within the nucleocapsid protein of Hantaan virus and establishment of cytotoxic T-cell clones about?
Although neutralizing antibodies against Hantaan virus (HTV) can protect hosts from viral infection, T-cell responses to HTV are also important in host defense against HTV. However, much less is known about cytotoxic T lymphocyte (CTL) responses to HTV. To identify CTL epitopes in the HTV nucleocapsid protein (NP), we selected 7 H-2K b -motif-fitting peptides. Of these peptides, 3 peptides (NP3, NP4, and NP7) were recognized by CTL responses derived from HTVimmunized mouse splenocytes. NP3 and NP4 peptides were also recognized by HTVimmunized splenocytes after secondary in vitro stimulation with the relevant peptide, but NP7 could not be recognized after in vitro stimulation. These results agree well with peptide immunization studies showing that peptidespecific CTL responses could be induced with NP3 and NP4 but not with NP7 peptide. Furthermore, CTL activity assay using targets, prepared to express the antigen (NP) endogenously, demonstrated that NP3 and NP4 peptides could be presented endogenously. CTL elicited with NP4 peptide retained some cross-reactivity and was difficult to long-term culture. However, NP3elicited CTL was very specific for NP3 peptide and was stable enough t
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- Author
- Park, Jong Myun; Cho, Sung Yoo; Hwang, Yu Kyeong; Um, Sung Hee; Kim, Woo Jung; Cheong, Hong Seok; Byun, Si Myung
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)
- Published
- 2000
- Language
- EN
- Field
- Medicine (Health Sciences)