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CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus by Skotnicka, Dorota; Steinchen, Wieland; Szadkowski, Dobromir; Cadby, Ian T.; Lovering, Andrew L.; Bange, Gert; SÃ ̧gaard-Andersen, Lotte is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Cyclic di-GMP (c-di-GMP) is a second messenger that modulates multiple responses to environmental and cellular signals in bacteria. Here we identify CdbA, a DNA-binding protein of the ribbon-helix-helix family that binds c-di-GMP in __Myxococcus xanthus__. CdbA is essential for viability, and its depletion causes defects in chromosome organization and segregation leading to a block in cell division. The protein binds to the __M. xanthus__ genome at multiple sites, with moderate sequence specificity; however, its depletion causes only modest changes in transcription. The interactions of CdbA with c-di-GMP and DNA appear to be mutually exclusive and residue substitutions in CdbA regions important for c-di-GMP binding abolish binding to both c-di-GMP and DNA, rendering these protein variants non-functional in vivo. We propose that CdbA acts as a nucleoid-associated protein that contributes to chromosome organization and is modulated by c-di-GMP, thus revealing a link between c-di-GMP signaling and chromosome biology.

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Author

Skotnicka, Dorota; Steinchen, Wieland; Szadkowski, Dobromir; Cadby, Ian T.; Lovering, Andrew L.; Bange, Gert; SÃ ̧gaard-Andersen, Lotte

Publisher

Nature Publishing Group; Springer Science and Business Media LLC; [London]: Nature Publishing Group, 2010-; Society for Mining, Metallurgy and Exploration Inc.; Research Square (ISSN 2041-1723)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)