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Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease by Xiuhua Wu; Zhimian Wang; Jing Shi; Xin Yu; Chaoran Li; Jinjing Liu; Fengchun Zhang; Hua Chen; Wenjie Zheng is a Medicine article available to read on EtoBox.
What is Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease about?
## Abstract ## Background Macrophages are key innate immune cells implicated in the pathogenesis of Behçet’s disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization. ## Methods BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4^+^T cell differentiation facilitated by HMDMs were measured by flow cytometry. Transcriptome analysis of BD and HC serum-stimulated HMDMs was conducted to identify differentially expressed genes. NF-κB signaling was examined using western blot to explore the mechanism of macrophage polarization induced by BD serum. ## Results BD serum-treated macrophages expressed a higher level of CD86, IL-12, and TNF-α and a lower level of CD163, which were compatible with the M1-like phenotype. Furthermore, BD serum-treated macrophages showed enhanced phagocytic capacity and promoted more Th1 cell differentiation. Sixty-one differentially expressed genes were identified between BD and HC serum-treated macrophages
Who reads Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease?
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- Author
- Xiuhua Wu; Zhimian Wang; Jing Shi; Xin Yu; Chaoran Li; Jinjing Liu; Fengchun Zhang; Hua Chen; Wenjie Zheng
- Publisher
- Springer Science and Business Media LLC
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)