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A Solid Phase Assay for the Protease of Human Immunodeficiency Virus by Ewald M. Wondrak; Terry D. Copeland; John M. Louis; Stephen Oroszlan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A Solid Phase Assay for the Protease of Human Immunodeficiency Virus about?

A solid phase assay for human immunodeficiency virus (HIV) protease using an immobilized substrate, Affi Gel 10-Gly-Gly-Gly-Gly-Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-[3H]Gly-OH has been devised. The Tyr-Pro bond of the substrate was hydrolyzed by the protease, releasing the radiolabeled cleavage product, Pro-Ile-Val-Gln-[3H]Gly-OH, into the supernatant. The pH optimum was found to be 6.0, and a high ionic strength was required for maximal activity. The solid phase assay is usable for convenient monitoring of purification procedures, and rapid screening of inhibitors of HIV protease.

Who reads A Solid Phase Assay for the Protease of Human Immunodeficiency Virus?

Author

Ewald M. Wondrak; Terry D. Copeland; John M. Louis; Stephen Oroszlan

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)