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Improved Chip Design for Integrated Solid-phase Microextraction in On-line Proteomic Sample Preparation by Jonas Bergkvist; Simon Ekström; Lars Wallman; Mikael Löfgren; György Marko-Varga; Johan Nilsson; Thomas Laurell is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Improved Chip Design for Integrated Solid-phase Microextraction in On-line Proteomic Sample Preparation about?
A recently introduced silicon microextraction chip (SMEC), used for on-line proteomic sample preparation, has proved to facilitate the process of protein identification by sample clean up and enrichment of peptides. It is demonstrated that a novel grid-SMEC design improves the operating characteristics for solid-phase microextraction, by reducing dispersion effects and thereby improving the sample preparation conditions. The structures investigated in this paper are treated both numerically and experimentally. The numerical approach is based on finite element analysis of the microfluidic flow in the microchip. The analysis is accomplished by use of the computational fluid dynamics-module FLOTRAN in the ANSYS software package. The modeling and analysis of the previously reported weir-SMEC design indicates some severe drawbacks, that can be reduced by changing the microextraction chip geometry to the grid-SMEC design. The overall analytical performance was thereby improved and also verified by experimental work. Matrix-assisted laser desorption/ionization mass spectra of model peptides extracted from both the weir-SMEC and the new grid-SMEC support the numerical analysis results. Fur
Who reads Improved Chip Design for Integrated Solid-phase Microextraction in On-line Proteomic Sample Preparation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jonas Bergkvist; Simon Ekström; Lars Wallman; Mikael Löfgren; György Marko-Varga; Johan Nilsson; Thomas Laurell
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)