Opening book details…
Can I read caCORRECT2: Improving the accuracy and reliability of microarray data in the presence of artifacts on EtoBox?
caCORRECT2: Improving the accuracy and reliability of microarray data in the presence of artifacts by Richard A Moffitt; Qiqin Yin-Goen; Todd H Stokes; R Mitchell Parry; James H Torrance; John H Phan; Andrew N Young; May D Wang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is caCORRECT2: Improving the accuracy and reliability of microarray data in the presence of artifacts about?
## Background In previous work, we reported the development of caCORRECT, a novel microarray quality control system built to identify and correct spatial artifacts commonly found on Affymetrix arrays. We have made recent improvements to caCORRECT, including the development of a model-based data-replacement strategy and integration with typical microarray workflows via caCORRECT's web portal and caBIG grid services. In this report, we demonstrate that caCORRECT improves the reproducibility and reliability of experimental results across several common Affymetrix microarray platforms. caCORRECT represents an advance over state-of-art quality control methods such as Harshlighting, and acts to improve gene expression calculation techniques such as PLIER, RMA and MAS5.0, because it incorporates spatial information into outlier detection as well as outlier information into probe normalization. The ability of caCORRECT to recover accurate gene expressions from low quality probe intensity data is assessed using a combination of real and synthetic artifacts with PCR follow-up confirmation and the affycomp spike in data. The caCORRECT tool can be accessed at the website: http://cacorrect.bme.
Who reads caCORRECT2: Improving the accuracy and reliability of microarray data in the presence of artifacts?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Richard A Moffitt; Qiqin Yin-Goen; Todd H Stokes; R Mitchell Parry; James H Torrance; John H Phan; Andrew N Young; May D Wang
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, [2000]-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2105)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)