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Apoptosis as an Underlying Mechanism in Lymphocytes Induced by Riboflavin and Ultraviolet Light by Hailiang Hu; Fei Xu; Wenle Yang; Jun Ren; Weihao Ge; Peng Yang is a Medicine article available to read on EtoBox.

What is Apoptosis as an Underlying Mechanism in Lymphocytes Induced by Riboflavin and Ultraviolet Light about?

Riboflavin plus UV light pathogen reduction technology (RF-PRT) is an effective method for inactivating donor-derived leukocytes (DDLs) in blood components. Literature data have shown that reactive oxygen species (ROS) increased in lymphocytes after RF-PRT treatment. Sustained high levels of ROS may abolish the endogenous antioxidant system, leading to damage to proteins, lipids, and nucleic acids, resulting in cell apoptosis. Nevertheless, whether riboflavin plus UV light can trigger leukocyte apoptosis remains obscure. In this study, a pool-and-split design, ABO/D-matched lymphocytes treated with RF-PRT or UV light or left untreated. After treatment, the level of ROS and intracellular calcium were measured in samples. Changes in the protein expression of cleaved PARP, Bax, and Bcl-2 and the activities of caspase-3 and caspase-9 were determined by immunoblot analysis or luminometer, respectively. Cell apoptosis was evaluated by flow cytometry. The effect of ROS on apoptosis was assessed. The RF-PRT treatment significantly augmented ROS production, intracellular calcium concentration. The pro-apoptotic proteins expression levels of Bax, but did not the anti-apoptotic protein Bcl-2,

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Author

Hailiang Hu; Fei Xu; Wenle Yang; Jun Ren; Weihao Ge; Peng Yang

Publisher

Elsevier BV

Published

2020

Language

EN

Field

Medicine (Health Sciences)