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Sherpa: A Macintosh-based Expert System for the Interpretation of Electrospray Ionization LC/MS and MS/MS data from Protein Digests by J. Alex Taylor; Kenneth A. Walsh; Richard S. Johnson is a Chemistry article available to read on EtoBox.

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A computer program, Sherpa, has been implemented to aid in the elucidation of electrospray ionization liquid chromatography/mass spectrometry (LC/MS) and tandem mass spectrometry data from protein digests. The program differs from existing analyis software in its ability to search concurrently against multiple plotein sequences, to search and compare two LC/MS files concurrently, to search for glycosylated and phosphorylated peptides, and to give a simple evaluation of the quality of a match between the data and a prediction. Details of the covalent structure of proteins are increasingly probed by using electrospray ionization mass spectrometry (ESI-MS)' to analyze their proteolytic digests. By directing the eluate from a high-performance, liquid chromatograph (HPLC) to an ESI mass spectrometer, the coupled technique, LC/MS,~-~ generates a very large amount of information from a single experiment in the form of hundreds of individual mass spectra. Although the LC/MS data can be collected in minutes, it may take many days to interpret them and correlate them to known sequences, depending on the complexity of the digest and the thoroughness of the investigation. Tandem mass spectrome

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Author

J. Alex Taylor; Kenneth A. Walsh; Richard S. Johnson

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

1996

Language

EN

Field

Chemistry (Physical Sciences)