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What is LDH Isoenzyme Separation by Electrophoresis about?
The document describes a procedure to separate and identify lactate dehydrogenase isoenzyme (LDH1-5) using agarose gel electrophoresis. There are five major LDH isoenzymes found in different tissues. Each isoenzyme has a different electrophoretic mobility based on its subunit composition. The procedure involves loading samples into an agarose gel, running electrophoresis, and then incubating the gel in a dye solution to visualize the bands of the separated isoenzymes. Observing the color and width of bands
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