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Ras-induced colony formation and anchorage-independent growth inhibited by elevated expression of Purα in NIH3T3 cells by Sharon M. Barr; Edward M. Johnson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Ras-induced colony formation and anchorage-independent growth inhibited by elevated expression of Purα in NIH3T3 cells about?
## Abstract Levels of Purα, a conserved, sequence‐specific single‐stranded DNA and RNA binding protein, fluctuate during the cell cycle, declining at the onset of S‐phase and peaking at mitosis. In early G1 Purα is associated with the hypophosphorylated form of the retinoblastoma protein, Rb. Microinjection of purified Purα into NIH3T3 cells arrests the cell cycle at either G1/S or G2/M checkpoints with distinct morphological consequences. Here we ask whether expression of Purα can affect colony formation and anchorage‐independent growth in __ras__‐transformed NIH3T3 cells. Two to five‐fold elevated levels of Purα in stably‐transfected cell lines retard entry into and progression through S phase in both __ras__‐transformed and non‐transformed cells. Purα significantly inhibits colony formation by __ras__‐transformed cells but not by non‐transformed cells. In addition, cells transfected to express Purα formed only about 1/5 the number of large colonies in soft agar as control‐transfected cells, demonstrating a marked inhibition of anchorage‐independent growth by Purα. Biochemical analysis of nuclear and cytoplasmic Purα proteins and confocal microscopic analysis of Purα location ind
Who reads Ras-induced colony formation and anchorage-independent growth inhibited by elevated expression of Purα in NIH3T3 cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sharon M. Barr; Edward M. Johnson
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)