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C7*9, a New Frequent C7 Allele Detected by an Allotype-Specific Monoclonal Antibody by Reinhard Würzner; Rüdiger Nitze; Otto Götze is a Medicine article available to read on EtoBox.

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Two sandwich ELISA for the quantitation of C7 in human plasma--one based on a monoclonal (MAb), the other performed with a polyclonal anti-C7 IgG--were established. Results from ELISA as well as from isoelectric focussing and immunoblotting with both antibodies revealed that the MAb is directed to an allotypic epitope on C7. About 6.7% of the plasma samples did not react with this MAb. Since these findings cannot be explained by the known alleles, the existence of a new C7 allele, C7\*9, was suggested, whose product C7-9 is not detected by the MAb. A gene frequency of 0.21 C7\*9 was assumed. When using conventional techniques C7\*9 is included in the frequency of C7\*1.

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Author

Reinhard Würzner; Rüdiger Nitze; Otto Götze

Publisher

S. Karger AG; Karger AG (ISSN 1012-8204)

Published

1990

Language

EN

Field

Medicine (Life Sciences)