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Compressive Nanomechanics of Opposing Aggrecan Macromolecules by Delphine Dean; Lin Han; Alan J. Grodzinsky; Christine Ortiz is a Medicine article available to read on EtoBox.

What is Compressive Nanomechanics of Opposing Aggrecan Macromolecules about?

In this study, we have measured the nanoscale compressive interactions between opposing aggrecan macromolecules in near-physiological conditions, in order to elucidate the molecular origins of tissue-level cartilage biomechanical behavior. Aggrecan molecules from fetal bovine epiphyseal cartilage were chemically end-grafted to planar substrates, standard nanosized atomic force microscopy (AFM) probe tips (R(tip) approximately 50 nm), and larger colloidal probe tips (R(tip) approximately 2.5 microm). To assess normal nanomechanical interaction forces between opposing aggrecan layers, substrates with microcontact printed aggrecan were imaged using contact mode AFM, and aggrecan layer height (and hence deformation) was measured as a function of solution ionic strength (IS) and applied normal load. Then, using high-resolution force spectroscopy, nanoscale compressive forces between opposing aggrecan on the tip and substrate were measured versus tip-substrate separation distance in 0.001-1M NaCl. Nanosized tips enabled measurement of the molecular stiffness of 2-4 aggrecan while colloidal tips probed the nanomechanical properties of larger assemblies (approximately 10(4) molecules). The

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Author

Delphine Dean; Lin Han; Alan J. Grodzinsky; Christine Ortiz

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0021-9290)

Published

2006

Language

EN

Field

Medicine (Health Sciences)