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Condensed states of nucleic acids. III. Ψ(+) and Ψ(−) Conformational transitions of DNA induced by ethanol and salt by Ruth Huey; Scott C. Mohr is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The conformational response of calf thymus DNA to solvent conditions altered by varying amounts of ethanol and NaCl has been monitored by circular dichroism (CD). These measurements, which extend over a much greater range of conditions than previously examined, reveal (above critical concentrations of ethanol and salt) a condensed form of the macro‐molecule with unusually large __positive__ ellipticity in the 250–300‐nm region [the Ψ(+) state]. Mere increase in NaCl concentration at constant 35% (v/v) concentration of ethanol suffices to convert such Ψ(+) samples—via a series of intermediate forms with CD spectra resembling those of A‐DNA, then B‐DNA—into Ψ(−) states having anomalously large __negative__ ellipticity similar to the well‐known Ψ(−) forms produced by above‐critical concentrations of poly‐(ethylene oxide) and salt. These ethanol/salt‐induced transitions are all completely reversible and can occur without formation of any visible precipitates. We suggest that they represent predominantly tertiary structural changes of B‐form DNA molecules analogous to the changes which occur in several other systems where Ψ(+) ⇌ Ψ(−) interconversion has been reported. A “ske

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Author

Ruth Huey; Scott C. Mohr

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0006-3525)

Published

1981

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)