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Sub-surface, Micrometer-scale Incisions Produced in Rodent Cortex Using Tightly-focused Femtosecond Laser Pulses by John Nguyen; Jillian Ferdman; Mingrui Zhao; David Huland; Shatha Saqqa; Jan Ma; Nozomi Nishimura; Theodore H. Schwartz; Chris B. Schaffer is a Medicine article available to read on EtoBox.

What is Sub-surface, Micrometer-scale Incisions Produced in Rodent Cortex Using Tightly-focused Femtosecond Laser Pulses about?

## Abstract ## Background and Objective Techniques that allow targeted, micrometer‐scale disruption in the depths of biological tissue, without affecting overlying structures or causing significant collateral damage, could potentially lead to new surgical procedures. We describe an optical technique to make sub‐surface incisions in __in vivo__ rodent brain and characterize the relationship between the cut width and maximum depth of these optical transections as a function of laser energy. ## Materials and Methods To produce cuts, high intensity, femtosecond laser pulses were tightly focused into and translated within the cortex, through a craniotomy, in anesthetized rodents. Imaging of stained brain slices was used to characterize cut width and maximum cutting depth. ## Results Cut width decreased exponentially as a function of depth and increased as the cube root of laser energy, but showed about 50% variation at fixed depth and laser energy. For example, at a laser energy of 13 μJ, cut width decreased from 158 ± 43.1 μm (mean ± standard deviation) to 56 ± 33 μm over depths of approximately 200–800 μm, respectively. Maximal cut depth increased logarithmically with laser energy, wi

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Author

John Nguyen; Jillian Ferdman; Mingrui Zhao; David Huland; Shatha Saqqa; Jan Ma; Nozomi Nishimura; Theodore H. Schwartz; Chris B. Schaffer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0196-8092)

Published

2011

Language

EN

Field

Medicine (Health Sciences)