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IgE-binding molecules (Mac-2/εBP) expressed by human eosinophils. Implication in IgE-dependent eosinophil cytotoxicity by Marie-José Truong; Valérie Gruart; Fu-Tong Liu; Lionel Prin; André Capron; Monique Capron is a scholarly article available to read on EtoBox.

What is IgE-binding molecules (Mac-2/εBP) expressed by human eosinophils. Implication in IgE-dependent eosinophil cytotoxicity about?

## Abstract Macrophage cell‐surface protein 2 (Mac‐2), a galactose specific S‐type lectin identified in inflammatory macrophages, presents a high degree of homology with the rat IgE‐binding protein (εBP). In the present study, we show by different experimental approaches that human eosinophils can express Mac‐2/εBP. Flow cytometry analysis revealed that a large proportion of eosinophilic patients expressing binding sites for IgE on their eosinophil membrane, were able to bind anti‐Mac‐2 monoclonal antibody (mAb). Northern blot performed with eosinophil RNA hybridized with the human Mac‐2 or εBP cDNA probes revealed that eosinophils presented a unique transcript at 1.2 kb. Immunoprecipitation of eosinophil extracts with anti‐Mac‐2 mAb revealed the presence of a molecule of 29 kDa corresponding to Mac‐2 protein, as well as one additional molecule of 15 kDa, absent from control alveolar macrophages. The function of these molecules was investigated in a radiolabeled IgE binding assay. Anti‐Mac‐2 mAb as well as galactose and lactose saccharides significantly inhibited the binding of radiolabeled human myeloma IgE protein to eosinophils. Moreover, the dose‐dependent inhibition by anti‐Ma

Author

Marie-José Truong; Valérie Gruart; Fu-Tong Liu; Lionel Prin; André Capron; Monique Capron

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0014-2980)

Published

1993

Language

EN