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Can I read DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation on EtoBox?
DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation by Aleksandra K. Adamczyk; Teun A. P. M. Huijben; Miguel Sison; Andrea Di Luca; Germán Chiarelli; Stefano Vanni; Sophie Brasselet; Kim I. Mortensen; Fernando D. Stefani; Mauricio Pilo-Pais; Guillermo P. Acuna is a Engineering article available to read on EtoBox.
What is DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation about?
An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control, and well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up nanofabrication methodology that fulfils almost all of these features. It enables the nanometric positioning of molecules and nanoparticles with stoichiometric control, and even the orientation of asymmetrical nanoparticles along predefined directions. However, orienting individual molecules has been a standing challenge. Here, we show how single molecules, namely, Cy5 and Cy3 fluorophores, can be incorporated in a DNA origami with controlled orientation by doubly linking them to oligonucleotide strands that are hybridized while leaving unpaired bases in the scaffold. Increasing the number of bases unpaired induces a stretching of the fluorophore linkers, reducing its mobility freedom, and leaves more space for the fluorophore to accommodate and find different sites for interaction with the DNA. Particularly, we explore the effects of leaving 0, 2, 4, 6, and 8 bases unpaired and find extreme orientations for 0 and 8 unpaired base
Who reads DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Aleksandra K. Adamczyk; Teun A. P. M. Huijben; Miguel Sison; Andrea Di Luca; Germán Chiarelli; Stefano Vanni; Sophie Brasselet; Kim I. Mortensen; Fernando D. Stefani; Mauricio Pilo-Pais; Guillermo P. Acuna
- Publisher
- American Chemical Society (ACS)
- Published
- 2022
- Language
- EN
- Field
- Engineering (Physical Sciences)