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Validation of a Targeted Gene Panel Sequencing for the Diagnosis of Hereditary Chronic Liver Diseases by G. Passignani; L. Ronzoni; I. Marini; F. Malvestiti; D. Marchelli; C. Bianco; S. Pelusi; D. Prati; L. Valenti is a Medicine article available to read on EtoBox.

What is Validation of a Targeted Gene Panel Sequencing for the Diagnosis of Hereditary Chronic Liver Diseases about?

Introduction: Sarcopenia is present in about 25-70% of patients with chronic liver diseases (CLDs), with higher rates in males. Understanding the mechanisms of CLD-related sarcopenia is mandatory for its management. Aim: We investigated the mechanism of sarcopenia in a murine model of liver fibrosis, focusing on sex-related differences, as well as the mechanisms involved in its resolution during liver regeneration. Materials and Methods: Liver fibrosis was induced in female and male mice by injecting increasing doses of carbon tetrachloride (CCl 4 , from 0.17 to 0.72 mL/Kg * bw) for 12 weeks. Muscle function and strength were evaluated by the grid hanging and the grip strength test. Mice were sacrificed after 6 and 12 weeks of CCl 4treatment, and after the 8-week washout period to study regeneration. H&E staining was used to calculate the cross-sectional area (CSA) of muscle quadriceps fibers. Muscle pAkt and p4EBP1 protein expression was assessed by western blot, mRNA expression of Musa, Atrogin-1, Murf-1, and Bnip3 by qRT-PCR. Results: Liver fibrosis was associated to a progressive loss of muscle strength in both sexes as demonstrated by the statistically significant reduction of

Who reads Validation of a Targeted Gene Panel Sequencing for the Diagnosis of Hereditary Chronic Liver Diseases?

Author

G. Passignani; L. Ronzoni; I. Marini; F. Malvestiti; D. Marchelli; C. Bianco; S. Pelusi; D. Prati; L. Valenti

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)