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Studies on the Nature of the Subcellular Localization of Lactate Dehydrogenase and Glyceraldehyde‐3‐phosphate Dehydrogenase in Chicken Skeletal Muscle by Ronald L. Melnick; H. O. Hultin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The elucidation of the subcellular localization of enzymes by the classical technique of homogenization followed by differential centrifugation is limited in that it is difficult to determine the effect of the severe disruptive procedures on the normal relationship of the enzymes to their subcellular environment. Attempts have been made to study this problem under less severe limitations; one of the approaches used has been the use of pressure on whole muscle tissue to extract the cellular fluids. In this report we introduce the concept of “comparative extraction” for evaluation of results obtained by this procedure. By comparing the efflux of enzymes of similar solubility and similar size and shape, it is possible to determine the minimal amount of the less easily extractable enzyme which cannot be removed due to compartmentation or binding to cellular particulate structures. Using this concept of “comparative extraction,” we show in this report that at least 35% of the lactate dehydrogenase of chicken breast muscle is restricted in its removal. The data do not definitely resolve the problem of whether the restriction is due to compartmentation of the enzyme within sub

Who reads Studies on the Nature of the Subcellular Localization of Lactate Dehydrogenase and Glyceraldehyde‐3‐phosphate Dehydrogenase in Chicken Skeletal Muscle?

Author

Ronald L. Melnick; H. O. Hultin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1973

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)