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Can I read TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap on EtoBox?
TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap by McShan, Andrew C. (author);Devlin, Christine A. (author);Morozov, Giora I. (author);Overall, Sarah A. (author);Moschidi, Danai (author);Akella, Neha (author);Procko, Erik (author);Sgourakis, Nikolaos G. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap about?
Chaperones Tapasin and TAP-binding protein related (TAPBPR) perform the important functions of stabilizing nascent MHC-I molecules (chaperoning) and selecting high-affinity peptides in the MHC-I groove (editing). While X-ray and cryo-EM snapshots of MHC-I in complex with TAPBPR and Tapasin, respectively, have provided important insights into the peptide-deficient MHC-I groove structure, the molecular mechanism through which these chaperones influence the selection of specific amino acid sequences remains incompletely characterized. Based on structural and functional data, a loop sequence of variable lengths has been proposed to stabilize empty MHC-I molecules through direct interactions with the floor of the groove. Using deep mutagenesis on two complementary expression systems, we find that important residues for the Tapasin/TAPBPR chaperoning activity are located on a large scaffolding surface, excluding the loop. Conversely, loop mutations influence TAPBPR interactions with properly conformed MHC-I molecules, relevant for peptide editing. Detailed biophysical characterization by solution NMR, ITC and FP-based assays shows that the loop hovers above the MHC-I groove to promote th
Who reads TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- McShan, Andrew C. (author);Devlin, Christine A. (author);Morozov, Giora I. (author);Overall, Sarah A. (author);Moschidi, Danai (author);Akella, Neha (author);Procko, Erik (author);Sgourakis, Nikolaos G. (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)