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Can I read A micromethod for base analysis of 32P-labeled oligoribonucleotides on EtoBox?
A micromethod for base analysis of 32P-labeled oligoribonucleotides by G. Volckaert; W. Fiers is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A micromethod for base analysis of 32P-labeled oligoribonucleotides about?
32P-Labeled RNA or an oligonucleotide is hydrolyzed to 3'-mononucleotides by means of a crude preparation containing ribonucleases T, and T,. The four mononucleotides are separated on a small polyethyleneimine plate. The method is very fast and simple, can conveniently be carried out on a large series of samples, and requires less than 50 dpm per analysis. tides are separated by micro-tic. Hydrolysis to 3'-nucleotides can be 1 To whom reprint requests should be addressed.
Who reads A micromethod for base analysis of 32P-labeled oligoribonucleotides?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- G. Volckaert; W. Fiers
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1977
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)