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Ion-exchange chromatography is an analytical technique used to separate ionic compounds. It works by exploiting ionic interactions between charged analytes and oppositely charged functional groups fixed to the chromatographic support. There are two main mechanisms - ion exchange, where analytes competitively bind to the stationary phase, and ion exclusion, where similarly charged analytes and stationary phase ions repel each other. Ion-exchange chromatography has been widely used since the 1950s to separate

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wandawd

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