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The Alkylphospholipid, Perifosine, Radiosensitizes Prostate Cancer Cells Both in Vitro and in Vivo by Yuanhong Gao; Hiromichi Ishiyama; Mianen Sun; Kathryn L Brinkman; Xiaozhen Wang; Julie Zhu; Weiyuan Mai; Ying Huang; Daniel Floryk; Michael Ittmann; Timothy C Thompson; E Brian Butler; Bo Xu; Bin S Teh is a Medicine article available to read on EtoBox.
What is The Alkylphospholipid, Perifosine, Radiosensitizes Prostate Cancer Cells Both in Vitro and in Vivo about?
## Background Perifosine is a membrane-targeted alkylphospholipid developed to inhibit the PI3K/Akt pathway and has been suggested as a favorable candidate for combined use with radiotherapy. In this study, we investigated the effect of the combined treatment of perifosine and radiation (CTPR) on prostate cancer cells __in vitro__ and on prostate cancer xenografts __in vivo__. ## Methods Human prostate cancer cell line, CWR22RV1, was treated with perifosine, radiation, or CTPR. Clonogenic survival assays, sulforhodamine B cytotoxity assays and cell density assays were used to assess the effectiveness of each therapy __in vitro__. Measurements of apoptosis, cell cycle analysis by flow cytometry and Western blots were used to evaluate mechanisms of action __in vitro__. Tumor growth delay assays were used to evaluate radiation induced tumor responses __in vivo__. ## Results __In vitro__, CTPR had greater inhibitory effects on prostate cancer cell viability and clonogenic survival than either perifosine or radiation treatment alone. A marked increase in prostate cancer cell apoptosis was noted in CTPR. Phosphorylation of AKT-T308 AKT and S473 were decreased when using perifosine treatm
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It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Yuanhong Gao; Hiromichi Ishiyama; Mianen Sun; Kathryn L Brinkman; Xiaozhen Wang; Julie Zhu; Weiyuan Mai; Ying Huang; Daniel Floryk; Michael Ittmann; Timothy C Thompson; E Brian Butler; Bo Xu; Bin S Teh
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2006-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1748-717X)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)