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Protein expression profiles in Saccharomyces cerevisiae during apoptosis induced by H2O2 by Francesca Magherini; Chiara Tani; Tania Gamberi; Anna Caselli; Laura Bianchi; Luca Bini; Alessandra Modesti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract We identified the proteins involved during apoptosis induced by H~2~O~2~ in __Saccharomyces cerevisiae__, and analyzed the global protein pattern by 2‐DE. We analyzed classical parameters of apoptosis such as chromatin condensation, DNA fragmentation, and morphology changes of cells. Exposure of yeast cells to nonphysiological doses of peroxides decreases the expression (or increases degradation) of enzymes involved in protection against oxidative stress. This leads the yeast cells to a reduction of their antioxidant defense and makes the cells more prone to apoptosis. In our data the down expression of peroxiredoxin II and GST I, could induce a perturbation of mitochondrial function with an alteration of permeability of the membrane leading to the mitochondria‐mediated apoptosis. Moreover, we identified a new spot of a classical glycolytic enzyme: the glyceraldehyde 3‐phosphate dehydrogenase during apoptosis. It is known that GAPDH is an extremely abundant glycolytic enzyme with multiple functions and that its overexpression is evident during apoptosis induced by a variety of stimuli. Our results confirm that it is a major intracellular messenger mediating apoptotic de
Who reads Protein expression profiles in Saccharomyces cerevisiae during apoptosis induced by H2O2?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Francesca Magherini; Chiara Tani; Tania Gamberi; Anna Caselli; Laura Bianchi; Luca Bini; Alessandra Modesti
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)