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Can I read Affinity chromatography of heme-binding proteins: An improved method for the synthesis of hemin-agarose on EtoBox?
Affinity chromatography of heme-binding proteins: An improved method for the synthesis of hemin-agarose by Ken Tsutsui; Gerald C. Mueller is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Affinity chromatography of heme-binding proteins: An improved method for the synthesis of hemin-agarose about?
Hemin was conjugated to aminoethyl-agarose with high efficiency using l,l'-carbonyldiimidazole as a condensing agent. The hemin-agarose was an efficient affinity resin for certain heme-binding proteins. It retained 4-5 mg of globin per milliliter of packed gel, whereas hemoglobin did not bind to the resin. The specificity of the adsorbent was further demonstrated by the selective binding of hemopexin from the whole sera of several species of a new hemebinding protein (HBP.93) from rabbit serum. Serum albumin, a major protein in serum which can also bind heme, did not bind to the adsorbent.
Who reads Affinity chromatography of heme-binding proteins: An improved method for the synthesis of hemin-agarose?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ken Tsutsui; Gerald C. Mueller
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1982
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)