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Functional Characterization of P53 Molecules Expressed in Human Squamous Cell Carcinomas of the Head and Neck by W. Andrew Yeudall; Judit Jakus; John F. Ensley; Keith C. Robbins is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Functional Characterization of P53 Molecules Expressed in Human Squamous Cell Carcinomas of the Head and Neck about?
Mutation of the p53 tumor suppressor gene has been demonstrated in a large proportion of human head and neck squamous cell carcinomas (HNSCCs) and has been assumed to play a role in the pathogenesis of these tumors, although no formal evidence of functional aberration has been demonstrated. In this study, we isolated cDNA clones encoding the entire p53 coding region from six human HNSCC cell lines that showed aberrant patterns of p53 expression in the parental cells, analyzed their nucleotide sequences, and characterized their function in vivo. cDNAs cloned from four cell lines harbored alterations within the p53 coding sequence (one missense mutation, one missense mutation plus in-frame deletion, one splice donor mutation, and a 1-nt insertion). HN30 cells, which contained wild-type p53 nucleotide sequences, showed a high constitutive level of protein expression. HN26 cells contained wild-type coding sequences but did not express the 53-kDa protein, although the mRNA was transcribed and a molecule of increased molecular mass (70 kDa) was observed by western blotting. Functional studies revealed that none of the four proteins encoded by mutant cDNAs were able to transactivate expre
Who reads Functional Characterization of P53 Molecules Expressed in Human Squamous Cell Carcinomas of the Head and Neck?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- W. Andrew Yeudall; Judit Jakus; John F. Ensley; Keith C. Robbins
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)
- Published
- 1997
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)