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Identification of novel Bach2 transcripts and protein isoforms through tagging analysis of retroviral integrations in B-cell lymphomas by Jinghua Liu; Annette Balle Sørensen; Bruce Wang; Matthias Wabl; Lade Anders Nielsen; Finn Skou Pedersen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification of novel Bach2 transcripts and protein isoforms through tagging analysis of retroviral integrations in B-cell lymphomas about?

## Abstract ## Background The __Bach2__ gene functions as a transcriptional repressor in B-cells, showing high expression level only before the plasma cell stage. Several lines of evidence indicate that Bach2 is a B-cell specific tumor suppressor. We here address patterns of insertional mutagenesis and expression of Bach2 is a murine retroviral model of B-cell lymphoma induction. ## Results We report that the __Bach2__ gene is a target of proviral integrations in B-cell lymphomas induced by murine leukemia virus. An alternative __Bach2__ promoter was identified within intron 2 and this promoter was activated in one of the tumors harboring proviral integration. The alternative promoter was active in both normal and tumor tissue and the tissue specificity of the two __Bach2__ promoters was similar. Three different alternatively used __Bach2__ terminal exons were identified to be located in intron 4. The inclusion of these exons resulted in the generation of __Bach2__ mRNA with open reading frames lacking the bZIP DNA binding domain present in the normal Bach2 protein, but retaining a partial BTB protein dimerization domain. Such Bach2 protein was excluded from the cell nucleus. ## Co

Who reads Identification of novel Bach2 transcripts and protein isoforms through tagging analysis of retroviral integrations in B-cell lymphomas?

Author

Jinghua Liu; Annette Balle Sørensen; Bruce Wang; Matthias Wabl; Lade Anders Nielsen; Finn Skou Pedersen

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)