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Convergence of two global transcriptional regulators on nitrogen induction of the stress‐acclimation gene nblA in the cyanobacterium Synechococcus sp. PCC 7942 by Ignacio Luque; Gérald Zabulon; Asunción Contreras; Jean Houmard is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Convergence of two global transcriptional regulators on nitrogen induction of the stress‐acclimation gene nblA in the cyanobacterium Synechococcus sp. PCC 7942 about?

Cyanobacteria respond to environmental stress conditions by degrading their phycobilisomes, the light harvesting complexes for photosynthesis. The expression of __nblA__, a key gene in this process, is controlled by the response regulator NblR in __Synechococcus__ sp. PCC 7942. Here we show that, under nitrogen stress, __nblA__ is also regulated by NtcA, the global regulator for nitrogen control. NtcA activation of __nblA__ was found to be nitrogen‐specific and did not take place under sulphur stress. Transcripts from the two major transcription start points (tsp) for the __nblA__ gene were induced in response to nitrogen and sulphur starvation. The most active one (__tspII__) required both NblR and NtcA to induce full __nblA__ expression under nitrogen starvation. NblR and NtcA bound __in vitro__ to a DNA fragment from the __nblA__ promoter region, suggesting that, under nitrogen stress, both NblR and NtcA activate the main regulated promoter (P__nblA__‐2) by direct DNA‐binding. The structure of P__nblA__‐2 differs from that of the canonical NtcA‐activated promoter and it is therefore proposed to represent a novel type of NtcA‐dependent promoter. We analysed expression patterns fr

Who reads Convergence of two global transcriptional regulators on nitrogen induction of the stress‐acclimation gene nblA in the cyanobacterium Synechococcus sp. PCC 7942?

Author

Ignacio Luque; Gérald Zabulon; Asunción Contreras; Jean Houmard

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)