Can I read AMPK: Methods and Protocols (Methods in Molecular Biology, 1732) on EtoBox?

AMPK: Methods and Protocols (Methods in Molecular Biology, 1732) by Dietbert Neumann; Benoît Viollet is a nonfiction available to read on EtoBox.

What is AMPK: Methods and Protocols (Methods in Molecular Biology, 1732) about?

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, __AMPK: Methods

Who reads AMPK: Methods and Protocols (Methods in Molecular Biology, 1732)?

Author

Dietbert Neumann; Benoît Viollet

Publisher

Springer New York : Imprint : Humana Press

Published

2018

Language

EN

ISBN

9781493975983

Category

nonfiction

Subjects

Science, Chemistry, Medical