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Can I read Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish on EtoBox?
Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish by Hans, Stefan (author);Zöller, Daniela (author);Hammer, Juliane (author);Stucke, Johanna (author);Spieß, Sandra (author);Kesavan, Gokul (author);Kroehne, Volker (author);Eguiguren, Juan Sebastian (author);Ezhkova, Diana (author);Petzold, Andreas (author);Dahl, Andreas (author);Brand, Michael (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish about?
## Abstract Conditional gene inactivation is a powerful tool to determine gene function when constitutive mutations result in detrimental effects. The most commonly used technique to achieve conditional gene inactivation employs the Cre/loxP system and its ability to delete DNA sequences flanked by two loxP sites. However, targeting a gene with two loxP sites is time and labor consuming. Here, we show Cre-Controlled CRISPR (3C) mutagenesis to circumvent these issues. 3C relies on gRNA and Cre-dependent Cas9-GFP expression from the same transgene. Exogenous or transgenic supply of Cre results in Cas9-GFP expression and subsequent mutagenesis of the gene of interest. The recombined cells become fluorescently visible enabling their isolation and subjection to various omics techniques. Hence, 3C mutagenesis provides a valuable alternative to the production of loxP-flanked alleles. It might even enable the conditional inactivation of multiple genes simultaneously and should be applicable to other model organisms amenable to single integration transgenesis.
Who reads Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hans, Stefan (author);Zöller, Daniela (author);Hammer, Juliane (author);Stucke, Johanna (author);Spieß, Sandra (author);Kesavan, Gokul (author);Kroehne, Volker (author);Eguiguren, Juan Sebastian (author);Ezhkova, Diana (author);Petzold, Andreas (author);Dahl, Andreas (author);Brand, Michael (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)