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Proteomic dissection of rice cytoskeleton reveals the dominance of microtubule and microfilament proteins, and novel compon ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Plant growth and maintenance 2 2.2 Isolation of cytoskeleton and cytoskeleton-bound polysome complex 2 2.3 Cytoskeleton enrichment and release of CBP complex 2 2.4 Analysis of cytoskeleton and CBP complex 2 2.5 Quantification and purity assessment of cytoskeleton-enriched proteins 2 2.6 Protein separation and trypsin digestion 3 2.7 LC-MS/MS analysis and protein identification 3 2.8 In-silico analysis of functional interaction network 3 2.9 Bioinformatics analyses 3 3 Results and discussion 3 3.1 Isolation, enrichment and purity assessment of cytoskeleton 3 3.2 Comparative cytoskeletal proteome profiling by gel-based and gel-free approaches 4 3.3 Identification of cytoskeletal proteins 5 3.4 Identification of cytoskeleton-associated proteins 5 3.5 Identification of hypothetical, uncharacterized protein and probable proteins 6 3.6 Physicochemical characteristics of cytoskeletal proteins 6 3.7 GO analysis of the cytoskeletal proteome 6 3.8 Cytoskeletal protein interactome network 7 3.9 Cross-species comparison

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nonfiction