About this Medicine article

A Monovalent Ion-selective Cation Current Activated by Noradrenaline in Smooth Muscle Cells of Rabbit Ear Artery by Q. Wang; R. C. Hogg; W. A. Large is a Medicine article available to read on EtoBox.

Membrane currents were recorded with the perforated-patch method with a low-chloride (35 mM) pipette solution in isolated smooth muscle cells of the rabbit ear artery. At a holding potential of -50 mV in potassium-free conditions spontaneous inward singlechannel currents were observed and noradrenaline evoked a noisy inward current, which appeared to be comprised of the spontaneous currents. The reversal potential (Vr) of the spontaneous channel and noradrenaline-induced current was not affected in anion-substitution experiments but Vr was altered when external Na + was replaced with choline or TRIS. The relationship between clamp potential and spontaneous single-channel current amplitude was linear and the mean unitary conductance was 28 pS. Caffeine, which releases calcium from the sarcoplasmic reticulum, and the calcium ionophore ionomycin activated the cation current and also blocked the response to noradrenaline. Spontaneous channel current activity and the noradrenaline-induced current were blocked when external NaCI was replaced with 89 mM CaC12. The response to noradrenaline was blocked by prazosin but was not affected by yohimbine and therefore the response is mediated by

Author

Q. Wang; R. C. Hogg; W. A. Large

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0031-6768)

Published

1993

Language

EN

Field

Medicine (Life Sciences)