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The uptake of 2-ketogluconate by Pseudomonas putida by G. De Torrontegui; R. Díaz; J. L. Cánovas is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is The uptake of 2-ketogluconate by Pseudomonas putida about?

The uptake of 2-ketogluconate is inducible in Pseudomonas putida: 2-ketogluconate, glucose, gluconate, glycerol and glycerate were each good nutritional inducers of this ability. 2-Ketogluconate uptake obeyed saturation kinetics (apparent Km in 2-ketogluconate-grown cells was 0.4 mM). 2-Ketogluconate was transported against a concentration gradient, apparently in an unchanged state, and the process required metabolic energy, all of which indicate an active transport system. A number of independently isolated mutants with deranged activity of a common glucose-gluconate uptake system were found to be also defective in 2-ketogluconate transport. Strains unable to transport 2-ketogluconate which grew readily on glucose and gluconate were also isolated. These results suggest that 2-ketogluconate transport is governed by at least two genetic elements: one which is also required to take up glucose and gluconate and another which appears to be specific for 2-ketogluconate transport. Similarly, glucose and gluconate transport appears to require at least one factor which is not necessary for 2-ketogluconate transport, as suggested by the lack of induction of the common glucose-gluconate upta

Who reads The uptake of 2-ketogluconate by Pseudomonas putida?

Author

G. De Torrontegui; R. Díaz; J. L. Cánovas

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Archie Comics (ISSN 0302-8933)

Published

1976

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)